Pharmacokinetics of Neostigmine and Glycopyrrolate

Eligibility Details: Inclusion Criteria: - Male or Female - Age 18-70 years Exclusion Criteria: - Previous adverse reaction or hypersensitivity to electrical stimulation, - Known sensitivity to neostigmine or glycopyrrolate, - History of mechanical obstruction of the GI or urinary tract, - Myocardial infarction within 6 months of trial, - Malignant and/or Uncontrollable Hypertension Defined by a blood pressure reading of 160/100 mmHg or higher with or without taking 3 or more different classes of anti-hypertensive medications, - Organ damage (heart & kidney) and/or TIA-CVA as a result of hypertension, - Known past history of coronary artery disease or bradyarrhythmia, - Symptomatic orthostatic hypotension - Deep brain stimulation - Pregnancy (women who are sexually active and of childbearing potential must utilize a method of contraception and agree to maintain a contraceptive method until completion of the study), - Lactating, nursing females - Inability to provide informed consent signaled by MoCA cognitive test score of 20 or less, - History of ingrown hair folliculitis, - Concurrent illness and fever, - Concurrent participation in a research study, - VA employee.